There are 20 essential proteins found in the human body. The amino groups are the building blocks of proteins. Hundreds and thousands of amino groups join together to from one protein. Proteins are the basic bio elements which perform all major functions of the body. Due advancements in the biotechnologies, the proteins are being used for several purposes. The proteins are altered in a way that they can be made more useful for the human body. Every day hundreds of tests and chemical reactions are tested to know whether a certain combination of proteins is beneficial for the body or not. For this purpose, it is essential that the proteins are marked with different chemicals so that they are easier to detect. Moreover, marking the proteins makes it easy to use them in a chemical test.
By labelling the proteins, it becomes easier to choose them for the test. There are many advantages of labelling the proteins with different chemicals.
Marking the proteins makes it easier to detect specific amino groups that are to be used in the chemical reaction. It saves both time and energy of the chemist. If the amino groups are not labelled using certain chemicals then it would become difficult to find out which amino groups are to be used. So labelling is a useful technique for the chemists.
Purification of proteins
The proteins that are to use up in a chemical reaction must be pure. Any impurity in the proteins will cause adverse reaction during the chemical testing and whole procedure will fail. So the proteins and the amino groups that are to be used in a chemical reaction must be completely pure. The labelling procedure of the amino groups and proteins make them clean and purified.
Immobilisation of proteins
During the labelling process, the proteins become immobile and don’t react with any substance. The reactive capacity of proteins is ceased by the chemicals that are used to labels. When the proteins are to be used for reactions these chemicals are drawn off and the protein can be used again for reaction. The chemicals help in restoring the original form of proteins in which they were extracted.
About Cy3 NHS ester
One of these chemicals is the Cyanine3 NHS ester. This is very reactive labelling agent. This is mostly used for proteins that are water soluble, the chemical is also used for labelling peptides, DNA and oligonucleotides. No co-solvents are to be used while you are using the Cyanine3 NHS ester. Some properties of the chemical are as under:
The Cyanine3 NHS ester is in red powder form. For being able to use it is mixed with water to form a liquid in which proteins can easily be dissolved.
The chemical is made up of five types of elements named as Carbon, chlorine, hydrogen, oxygen, and nitrogen. Like Cy3 NHS ester other similar chemicals are used for labelling proteins namely Cy3 PicolylAzide and Cy3 Tetrazine.
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